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    细胞微流体工作站VenaFlux (VenaFlux Platforms)是Cellix公司目前*受客户欢迎的一套系统,提供在活细胞水平上微流体活性筛选的完整解决方案。操作简单,分析效率高,可大大提高药品的开发和筛选效率。

    VenaFlux 系统是**个能进行连续流体细胞分析的半自动化微流体分析系统。配合使用Cellix生物芯片和细胞分析软件,VenaFlux 系统可检测粘附到微毛细管上的细胞,微毛细管中覆盖抗体或者培养有内皮细胞,模拟生理流动产生一定的剪切力。

    VenaFlux 系统操作简单,可减少耗时耗钱的动物模型实验,获得特异性强,准确性高,重复性好的结果。

    VenaFlux 流体细胞分析仪产品特点:

    ? 广泛使用于各种细胞悬浮液;

    ? 生物芯片可选择用光学显微镜做细节研究;

    ? 微流体泵可方便的控制流体剪切力在0.05到20dyne/cm2范围内;

    ? 在分析中可调整剪切力;

    ? 流体速度可控制在pL/min到mL/min;

    ? 可做nL到mL 的样品分析;

    ? 使用DucoCell 分析软件(DucoCell Analysis Software),可简单快速的调节好显微镜的各项参数;

    ? 统计分析生成的图表(如:细胞粘附性VS.剪切力)可方便的导入Excel文档;

    ? 电子机械臂移动生物芯片,清洗细微组件和稀释细胞样品;

    ? VenaFluxTM 系统的软件同步调节泵,翻译阶段筛选,图像采集,样品移动和流体控制,各项操作界面均是用户友好型,系统先进又易于操作;

    细胞微流体工作站 (VenaFlux Platforms)主要技术参数:

    Microscope Axiovert 40:

    Contrast option: Brightfield, Phase, Varel.

    Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan

    Eyepieces: E-PL 10x/20 Br., 10x/20 Br. foc.

    Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.

    Mirus 1.0 Nanopump

    Specifications: Shear Stress Range of 0.05–20 dyne/cm2,

    Volumetric Flow Rates 100nL–20μL/minute (100μL syringe),

    Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s;

    Flow Direction Reversible;

    Sample Volume Aspiration Accuracy ±1% ;

    Shear Stress Accuracy ±0.5%;

    Sample Volume Aspiration Precision < 1%.

    Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.

    Microscope Cage incubator:

    Capabilities: Temperature control ± 0.10C, CO2 and humidity module.

    Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.

    Deltapix DP 200 USB 2.0 camera

    Specifications: 1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2" format, Pixel 5.2 μm x 5.2 μm,

    Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.

    Dell Optiplex 745 PC computer

    Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;

    Objective Imaging stage control hardware; 19” LCD monitor.

    Windows XP Professional SP2;

    DeltaPix Viewer Software;

    FlowAssay and DucoCell software preinstalled.

    FlowAssay Software.

    Capabilities: Execution of preset protocols.

    Intuitive stepwise protocol interface with steps hierarchy and status control.

    DucoCell software

    Specifications: Automatic Counting and Detection;

    Morphological parameters analyzed automatically(Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis);

    Cell sorting and filtering;

    Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.

    Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).

    File Formats analyzed*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.

    Vena8 biochips pack of 10

    Specifications: Minimum Sample Volume 3 μL;

    Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2.

    Number of channels per biochip is 8;

    Volume of each channel is 0.8μL.

    Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).

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